Dissolving Uncharged Peptides

Dissolving Hydrophobic/Uncharged Peptides

  1. Dissolve peptides in a minimal amount of strong solvents such as Dimethylformamide (DMF), Acetonitrile (ACN), isopropyl alcohol, ethanol, acetic acid, 4-8M guanidine hydrochloride or urea, and Dimethylsulfoxide (DMSO) (if the sequence does not contain C, W or M).
  2. Slowly add (drop wise) the prepared peptide solution to a stirred aqueous buffer solution.
  3. If the resulting peptide solution begins to show turbidity, it might have reached the solubility limit and it will be futile to proceed. Note: the initial solvent of choice should be compatible with the experiment.