Determining Peptide Solubility Characteristics – Net Charge
- Assign a value of -1 to each acidic residue (D, E, and C-terminal COOH)
- Assign a value of +1 to each basic residue (K, R and the N-terminal NH2)
- Assign a value of +1 to each H residue at pH<6 and zero at pH >6.
- Count the total number of charges of the peptide at pH 7 (all D, E, K, R, C-terminal COOH, and C-terminal NH2).
- Calculate the overall net charge of the peptide