Fluorophores Absorption and Emission Data
The table lists the properties of the most commonly used fluorophore for peptide labeling of your interest in the life sciences. The fluorophores absorbance wavelength from 325nm to 743nm (from blue color to red color), the emission wavelength from 386nm to 770nm.
| Fluorophore Dyes | Ex (nm) | Em (nm) |
|---|---|---|
| Hydroxycoumarin | 325 | 386 |
| Dansyl | 340 | 578 |
| 7-Amino-4-methylcoumarin | 351 | 430 |
| methoxycoumarin | 360 | 410 |
| Alexa fluor | 345 | 442 |
| aminocoumarin | 350 | 445 |
| AMC | 345 | 445 |
| Dabcyl | 453 | – |
| Cy2 | 490 | 510 |
| FAM | 495 | 517 |
| Alexa fluor 488 | 494 | 517 |
| Fluorescein FITC | 495 | 519 |
| Alexa fluor 430 | 430 | 545 |
| 5-Carboxyfluorescein (5-FAM) | 492 | 518 |
| Alexa fluor 532 | 530 | 555 |
| HEX | 535 | 556 |
| 5-TAMRA | 542 | 568 |
| Cy3 | 550 | 570 |
| TRITC | 547 | 572 |
| Alexa fluor 546 | 556 | 573 |
| Alexa fluor 555 | 556 | 573 |
| Dansyl | 340 | 578 |
| R-phycoerythrin (PE) | (489) | 565 |
| Rhodamine Red-X | 560 | 580 |
| Tamara | 565 | 580 |
| Cy3 | (512) | 550 |
| Cy3.5 | 581 | 596 |
| Rox | 575 | 602 |
| Alexa fluor 568 | 578 | 603 |
| Texas Red | 589 | 615 |
| Alexa fluor 594 | 590 | 617 |
| Alexa fluor | 621 | 639 |
| Alexa fluor 633 | 650 | 668 |
| Cy5 | (625) | 650 |
| Alexa fluor 660 | 663 | 690 |
| Cy5.5 | 675 | 694 |
| TruRed | 490; 675 | 695 |
| Alexa fluor 680 | 679 | 702 |
| Cy7 | 743 | 767 |
