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Home / Peptide Resources / Peptide Modifications / Fluorescent Modifications
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Peptide Resources

  • Peptide Modifications
    • Antibody Modifications
    • Fluorescent Modifications
    • FRET Peptides
    • Peptide Cyclization
    • Standard Modifications
  • Peptide Handling
    • Dissolving Charged Peptides
    • Dissolving Peptides
    • Dissolving Uncharged Peptides
    • Lyophilized Peptides Storage
    • Peptide Solubility
    • Peptide Stability and Degradation
  • Fluorophore Properties
  • Design Peptide Antigen
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Fluorescent Modifications

Fluorescent Peptide Modifications – Dyes and Quenchers

AMC (7-Amino-4-methyl-coumarin)

UV-excitable dye, used in enzyme assays using cuvettes or flow cytometry.

AMC (7-Amino-4-methyl-coumarin)

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
AMC 353 nm 422 nm 19.000

Cy3, Cy5

Cy3, Cy5 are dyes with extremely high extinction coefficients and fluorescence. Thus, they are especially suitable for very sensitive localization assays of peptides in cells. Their disadvantage is the high instability of the molecules under peptide synthesis conditions. Therefore the yields are comparatively low.

Cy3, Cy5

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
Cy™3 550 nm 570 nm 150.000
Cy™5 650 nm 670 nm 250.000

Dabcyl

Dabcyl is a non-fluorescent dye predominantly used as a quencher for other fluorophores (esp. Fluorescent type dyes, EDANS.). If Dabcyl is coupled to a peptide in close proximity to a fluorophore, it absorbs the emitted light of the fluorophore. Enlarging this distance (i.e. by enzymatic cleavage of the peptide) results in excitation of the fluorophore with an emission signal that can be detected.

Dabcyl

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
Dabcyl 453 nm none 32.000

Dansyl

Dansyl is also used as a fluorophore quencher. Unlike Dabcyl, it inherits own fluorescence and thus might not be as useful for highly sensitive assays.

Dansyl

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
Dansyl 335 nm 526 nm 4.600

2,4-Dinitrophenyl (DNP)

2,4-Dinitropheny is a non-fluorescent dye that can be used as a fluorophore quencher (see Dabcyl for more details).

2,4-Dinitrophenyl (DNP)

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
DNP 348 nm none 18.000

DNP-Lysine

DNP-Lysineis a non-fluorescent dye that can be used as a fluorophore quencher, for custom peptide (see Dabcyl for more details).

DNP-Lysine

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
DNP-Lysine 348 nm none 18.000

EDANS (5-((2-aminoethyl)amino)napthalene-1-sulfonic acid)

EDANS is a commonly used dye in FRET (fluorescence resonance energy transfer) peptides in combination with Dabcyl as quencher.

EDANS (5-((2-aminoethyl)amino)napthalene-1-sulfonic acid)

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
EDANS 335 nm 493 nm 5.900

Fluorescent

Fluorescent is the commonly used fluorescent dye in confocal laser-scanning microscopy and flow cytometry applications.

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
Fluorescent 495 nm 520 nm 83.000

NBD (7-nitrobenz-2-oxa-1, 3-diazole)

NBD is a fluorescent dye, used for amine modification.

NBD (7-nitrobenz-2-oxa-1, 3-diazole)

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
NBD 486 nm 543 nm 27.000

p-Nitro-Aniline

p-Nitro-Aniline is a chromogen used as colorimetric enzyme substrate in many standard enzyme assays in cuvettes.

p-Nitro-Aniline


Rhodamine B

Rhodamine B represents one among a numerous range of rhodamine dyes, used in fluorescent peptide assays.

Rhodamine B

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
Rhodamine 550 nm 580 nm 90.000

Tamra

Tamra is the most commonly used rhodamine dye in fluorescence assays.

Tamra

Dye Excitation maximum Emission maximum Molar Extinction Coefficient
Tamra 544 nm 576 nm 90.000
Home / Peptide Resources / Peptide Modifications / Fluorescent Modifications
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