FRET-Fluorescense Resonance Energy Transfer Peptide
FRET stands for fluorescence resonance energy transfer, or Frster resonance energy transfer. FRET is a mechanism describing excited state energy transfer from the initially excited donor to an acceptor. The donor molecules typically emit at shorter wavelengths that overlap with the absorption of acceptors. The process is a distance-dependent interaction between the electronic excited states of two molecules without emission of a photon.
FRET can be used for conformational investigation of peptide CRO folding. FRET peptides are widely used as suitable substrates in enzyme studies, such as:
- Functional characterization of peptidases / proteases / kinases / phosphatases
- Kinetic characterization of peptidases / proteases / kinases / phosphatases
- Screening and detection of new proteolytic enzyme.
Common fluorescent pairs used in FRET peptide:
- Abz/Tyr(3-NO2)
- EDANS or DABCYL
